Enzyme immunoassays

enzyme immunoassays

Know in one minute about Enzyme immunoassay Enzyme immunoassay is an important technique for the detection of proteins, hormones, peptides, etc. EIA determines the sample by using enzyme-linked antibodies. Horseradish Peroxidase, alkaline phosphatase is used for labeling antigens or antibodies because they have chromogenic substrates. Enzyme immunoassay performs both qualitative and quantitative tests. We can … Read more

What is an EcoRI restriction enzyme?

EcoRI restriction enzyme

Know in one minute about EcoRI Restriction Enzyme EcoRI Restriction Enzyme is a type II restriction endonuclease. It was derived from the bacteria Escherichia coli RY13. It is the first enzyme to have been derived. The recognition sequence of this enzyme discovered in 1972 is 5′-GAATTC-3′. It consists of four nucleotide bases namely guanine, adenine, … Read more

Radioimmunoassay: Define with simple diagrams


Know in one minute about Radioimmunoassay Radioimmunoassay is used to determine the concentration of Ag present in the patient sample. It utilizes the radioactive isotopes to label antigens. Two types of RIA were found first is double antibody RIA and second is coated tube RIA. RIA technique based on the competition binding of labeled Ag … Read more

How to detect proteins by Western blotting

Western blotting

Know in one minute about Western blotting The western blotting technique was introduced by Towbin in 1979. The main application behind this technique is the determination of molecular weight. It has become an essential tool to monitor the mutations in proteins and post-translational changes associated with the protein. The first step to immunoblotting is to … Read more

Where do light-dependent reactions occur?

Where do light dependent reactions occur

Where do light-dependent reactions occur? The light reactions occur in the grana or thylakoid membrane of chloroplast and Involve processes that resemble mitochondrial electron Transport and oxidative phosphorylation. In photosynthetic prokaryotes, which lack Chloroplasts, the light reactions take place in the cell’s plasma membrane or in chromatophores. The photosynthesis process takes place in the chloroplast. … Read more

C value paradox

C value paradox

Know in one minute about C value paradox The c value paradox is the developing form of C value. C value is considered as the number of DNA base pairs present in the haploid set of the genome. C value phenomenon is the Complexity of an organism that is increased if the number of DNA … Read more

What is bioaugmentation?


Know in one minute about Bioaugmentation Bioaugmentation is a process in which specialized microorganisms are added to perform biodegradation of recalcitrant organic pollutants (present in soil and groundwater).  It is designed to reduce the contamination level. Increases the active microbes and microorganisms level in the treatment environment.  The contaminants are transformed into less harmful substances … Read more

What is G3P or Glyceraldehyde-3-phosphate?


Know in one minute about G3P or Glyceraldehyde – 3 – phosphate G3P is an important intermediate in respiration and photosynthesis. Also called triose phosphate. It is a 3-carbon sugar molecule. Consists of 3-carbon, one side phosphate group attached and the other side aldehyde group attached. Is an aldotriose that plays important role in glycolysis, … Read more